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r d systems 1433 op 050  (R&D Systems)


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    R&D Systems r d systems 1433 op 050
    R D Systems 1433 Op 050, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+opn+protein/Recombinant+Human+Osteopontin+(OPN)+Protein/pmc12985369-491-152-152
    Average 95 stars, based on 68 article reviews
    r d systems 1433 op 050 - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Recombinant:

    Article Title: Alcohol Inhibits Osteopontin-dependent Transforming Growth Factor-β1 Expression in Human Mesenchymal Stem Cells
    Article Snippet: .. Recombinant human OPN protein was obtained from R&D Systems (Minneapolis, MN). .. The αvβ3 integrin antibody, CD44 antibody, and S p -adenosine 3′,5′-cyclic monophosphorothioate triethylammonium salt hydrate (SP8) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).

    Article Title: Osteopontin mediates glioblastoma-associated macrophage infiltration and is a potential therapeutic target
    Article Snippet: .. Recombinant human OPN protein (10 ng/ml in most conditions) and its neutralizing antibody as well as integrin αvβ5 antibody (10 μg/ml) were purchased from R&D Systems, and recombinant human CCL2 (10 ng/ml) was from Peprotech. ..

    Article Title: The osteopontin-CD44 axis in hepatic cancer stem cells regulates IFN signaling and HCV replication
    Article Snippet: Huh7 cells, JFH-1-Huh7 cells, Huh7.5 cells, and MH14C cells were maintained in DMEM (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (Thermo Fisher Scientific), 1% L-glutamine (Thermo Fisher Scientific), and 1% penicillin/streptomycin (Thermo Fisher Scientific) in a humidified atmosphere of 5% CO 2 at 37 °C. .. Recombinant Human OPN protein was purchased from R&D Systems (Minneapolis, MN). .. Recombinant IFN-α, MG132, BIO, and MeBIO were purchased from Sigma-Aldrich (St. Louis, MO).

    Article Title: Biomimetic matrices for rapidly forming mineralized bone tissue based on stem cell-mediated osteogenesis
    Article Snippet: .. Recombinant human OPN protein was purchased from R&D systems and human OC fragment 1–49 was purchased from Sigma-Aldrich. .. Different concentrations of OC and OPN (Fig. ) were combined to 100 μl of chilled purified bovine type I collagen solution (PureCol: Advanced BioMatrix, San Diego, CA) at a final concentration of 3 mg/ml with gentle swirling. pH of mixture was adjusted to 7.2–7.6 using sterile 0.1 M NaOH (Sigma-Aldrich, St. Louis, MO).

    Article Title: Synergistic effect of extracellularly supplemented osteopontin and osteocalcin on stem cell proliferation, osteogenic differentiation, and angiogenic properties.
    Article Snippet: Department of Biomedical Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York Department of Bioengineering, iBB – Institute of Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Lisboa, Portugal The Discoveries Centre for Regenerative and Precision Medicine, Lisbon Campus, Instituto Superior Técnico, Universidade de Lisboa, Lisboa, Portugal

    Article Title: Colloidally Assembled Zinc Ferrite Magnetic Beads: Superparamagnetic Labels with High Magnetic Moments for MR Sensors.
    Article Snippet: Magnetic particles are widely used as labels in magnetoresistive (MR) sensors.. To use magnetic particles as labels, several important characteristics should be considered, such as superparamagnetism, a high magnetic moment per particle (m), facile surface functionalization and biomolecule immobilization, colloidal stability, and analyte specificity.. Described in this paper is the preparation of magnetic labels with a high m using colloidal assemblies of superparamagnetic zinc ferrite nanoparticles (ZFNPs, ~9 nm).

    Cell Culture:

    Article Title: Synergistic effect of extracellularly supplemented osteopontin and osteocalcin on stem cell proliferation, osteogenic differentiation, and angiogenic properties.
    Article Snippet: Department of Biomedical Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York Department of Bioengineering, iBB – Institute of Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Lisboa, Portugal The Discoveries Centre for Regenerative and Precision Medicine, Lisbon Campus, Instituto Superior Técnico, Universidade de Lisboa, Lisboa, Portugal



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    The decidua is rich <t>in</t> <t>COL4A1,</t> <t>OPN</t> and HA. (A, B) Compared the expression of COL4A1 (A) and OPN (B) in endometrium (n=5) and decidua (n=5) by Immunohistochemistry. Scale bar, 100 μm and 50 μm. (C) Compared the expression of HA in endometrium (n=8) and decidua (n=8) by Alcian blue staining assays. Scale bar, 100 μm. (D) The levels of COL4A1, OPN and HA from the culture supernatant of DSCs (n=6) or ESCs (n=6) were evaluated by ELISA. The data are presented as the mean ± SEM; two-tailed Student’s t -test; ** P < 0.01, **** P < 0.0001.
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    Image Search Results


    The decidua is rich in COL4A1, OPN and HA. (A, B) Compared the expression of COL4A1 (A) and OPN (B) in endometrium (n=5) and decidua (n=5) by Immunohistochemistry. Scale bar, 100 μm and 50 μm. (C) Compared the expression of HA in endometrium (n=8) and decidua (n=8) by Alcian blue staining assays. Scale bar, 100 μm. (D) The levels of COL4A1, OPN and HA from the culture supernatant of DSCs (n=6) or ESCs (n=6) were evaluated by ELISA. The data are presented as the mean ± SEM; two-tailed Student’s t -test; ** P < 0.01, **** P < 0.0001.

    Journal: Frontiers in Immunology

    Article Title: Decidual stromal cells drive CD16 + macrophages towards an immunoregulatory phenotype via extracellular matrix-adhesion molecule interaction during early pregnancy

    doi: 10.3389/fimmu.2025.1747323

    Figure Lengend Snippet: The decidua is rich in COL4A1, OPN and HA. (A, B) Compared the expression of COL4A1 (A) and OPN (B) in endometrium (n=5) and decidua (n=5) by Immunohistochemistry. Scale bar, 100 μm and 50 μm. (C) Compared the expression of HA in endometrium (n=8) and decidua (n=8) by Alcian blue staining assays. Scale bar, 100 μm. (D) The levels of COL4A1, OPN and HA from the culture supernatant of DSCs (n=6) or ESCs (n=6) were evaluated by ELISA. The data are presented as the mean ± SEM; two-tailed Student’s t -test; ** P < 0.01, **** P < 0.0001.

    Article Snippet: M2φ were treated with exogenous COL4A1 (10 ug/mL; Proteintech, Ag34402), OPN (1 ug/mL; ABclonal, RP00989), and HA (High Molecular Weight; 50μM, 100μM; R&D system, GLR002) for 48 hours.

    Techniques: Expressing, Immunohistochemistry, Staining, Enzyme-linked Immunosorbent Assay, Two Tailed Test

    Exogenous COL4A1, OPN and HA promote development of immunomodulatory CD16 + Mφ. (A, B) After 48 hours of stimulation with exogenous COL4A1 (10 ug/mL), OPN (1 ug/mL) or vehicles, the expression levels of CD16 on U937-induced Mφ ( A ; n=5) and the polarization-related molecules CD86, CD209 and CD206 on CD16 - or CD16 + Mφ ( B ; n=5) were detected by flow cytometry. (C, D) After 48 hours of stimulation with exogenous HA (0, 50, 100 μM), the expression levels of CD16 on U937-induced Mφ ( C ; n=4) and CD86, CD209 and CD206 on CD16 - or CD16 + Mφ ( D ; n=4) were detected by flow cytometry. The data are presented as the mean ± SEM; oneway ANOVA test or two-tailed Student’s t-test; *P < 0.05, **P < 0.01, ****P< 0.0001, NS, no significant difference.

    Journal: Frontiers in Immunology

    Article Title: Decidual stromal cells drive CD16 + macrophages towards an immunoregulatory phenotype via extracellular matrix-adhesion molecule interaction during early pregnancy

    doi: 10.3389/fimmu.2025.1747323

    Figure Lengend Snippet: Exogenous COL4A1, OPN and HA promote development of immunomodulatory CD16 + Mφ. (A, B) After 48 hours of stimulation with exogenous COL4A1 (10 ug/mL), OPN (1 ug/mL) or vehicles, the expression levels of CD16 on U937-induced Mφ ( A ; n=5) and the polarization-related molecules CD86, CD209 and CD206 on CD16 - or CD16 + Mφ ( B ; n=5) were detected by flow cytometry. (C, D) After 48 hours of stimulation with exogenous HA (0, 50, 100 μM), the expression levels of CD16 on U937-induced Mφ ( C ; n=4) and CD86, CD209 and CD206 on CD16 - or CD16 + Mφ ( D ; n=4) were detected by flow cytometry. The data are presented as the mean ± SEM; oneway ANOVA test or two-tailed Student’s t-test; *P < 0.05, **P < 0.01, ****P< 0.0001, NS, no significant difference.

    Article Snippet: M2φ were treated with exogenous COL4A1 (10 ug/mL; Proteintech, Ag34402), OPN (1 ug/mL; ABclonal, RP00989), and HA (High Molecular Weight; 50μM, 100μM; R&D system, GLR002) for 48 hours.

    Techniques: Expressing, Flow Cytometry, Two Tailed Test

    CD16 + Mφ in the co-culture system are suppressed with the inhibition of COL4A1, OPN and HA in DSCs. (A) DSCs were transfected by plasmid of siRNA targeting COL4A1 (si COL4A1 ; n=3), SPP1 (si SPP1 ; n=3) or control plasmids (n=3) for 72 hours and the efficacy was verified by RT-qPCR. (B-D) After co-cultured with COL4A1 -silenced DSCs, SPP1 -silenced DSCs or control DSCs for 48 hours, CD16 expression of U937-induced Mφ ( B, C ; n=4) and the polarization markers (CD86, CD209 and CD206) of CD16 - or CD16 + macrophages ( D ; n=4) were explored by flow cytometry. (E, F) After co-cultured with 4-MU (a hyaluronic Acid synthesis inhibitor, 500 μM) or vehicle treated DSCs for 48 hours, CD16 expression of U937-induced Mφ ( E ; n=6) and the polarization markers of CD16 - or CD16 + macrophages ( F ; n=6) were explored by flow cytometry. The data are presented as the mean ± SEM; oneway ANOVA test or two-tailed Student’s t -test; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, ns, NS significant difference.

    Journal: Frontiers in Immunology

    Article Title: Decidual stromal cells drive CD16 + macrophages towards an immunoregulatory phenotype via extracellular matrix-adhesion molecule interaction during early pregnancy

    doi: 10.3389/fimmu.2025.1747323

    Figure Lengend Snippet: CD16 + Mφ in the co-culture system are suppressed with the inhibition of COL4A1, OPN and HA in DSCs. (A) DSCs were transfected by plasmid of siRNA targeting COL4A1 (si COL4A1 ; n=3), SPP1 (si SPP1 ; n=3) or control plasmids (n=3) for 72 hours and the efficacy was verified by RT-qPCR. (B-D) After co-cultured with COL4A1 -silenced DSCs, SPP1 -silenced DSCs or control DSCs for 48 hours, CD16 expression of U937-induced Mφ ( B, C ; n=4) and the polarization markers (CD86, CD209 and CD206) of CD16 - or CD16 + macrophages ( D ; n=4) were explored by flow cytometry. (E, F) After co-cultured with 4-MU (a hyaluronic Acid synthesis inhibitor, 500 μM) or vehicle treated DSCs for 48 hours, CD16 expression of U937-induced Mφ ( E ; n=6) and the polarization markers of CD16 - or CD16 + macrophages ( F ; n=6) were explored by flow cytometry. The data are presented as the mean ± SEM; oneway ANOVA test or two-tailed Student’s t -test; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, ns, NS significant difference.

    Article Snippet: M2φ were treated with exogenous COL4A1 (10 ug/mL; Proteintech, Ag34402), OPN (1 ug/mL; ABclonal, RP00989), and HA (High Molecular Weight; 50μM, 100μM; R&D system, GLR002) for 48 hours.

    Techniques: Co-Culture Assay, Inhibition, Transfection, Plasmid Preparation, Control, Quantitative RT-PCR, Cell Culture, Expressing, Flow Cytometry, Two Tailed Test

    Patients RM exhibit weakened interactions between DSCs and CD16 + dMφ. (A) Compared the expression of COL4A1 in decidua from women with normal pregnancy (NP, n=8) or recurrent miscarriage (RM, n=8) by Immunohistochemistry. Scale bar, 50 μm. (B) Compared the expression of OPN in decidua from women with NP (n=10) or RM (n=10) by Immunohistochemistry. Scale bar, 50 μm. (C) Compared the expression of HA in decidua from women with NP (n=8) or RM (n=8) by Alcian blue staining assays. Scale bar, 50 μm. (D) Levels of CD16, CD86, CD206 and CD209 in CD45 + CD14 + dMφ from women with NP (n=7) or RM (n=7) by flow cytometry assays. The data are presented as the mean ± SEM; two-tailed Student’s t -test; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Journal: Frontiers in Immunology

    Article Title: Decidual stromal cells drive CD16 + macrophages towards an immunoregulatory phenotype via extracellular matrix-adhesion molecule interaction during early pregnancy

    doi: 10.3389/fimmu.2025.1747323

    Figure Lengend Snippet: Patients RM exhibit weakened interactions between DSCs and CD16 + dMφ. (A) Compared the expression of COL4A1 in decidua from women with normal pregnancy (NP, n=8) or recurrent miscarriage (RM, n=8) by Immunohistochemistry. Scale bar, 50 μm. (B) Compared the expression of OPN in decidua from women with NP (n=10) or RM (n=10) by Immunohistochemistry. Scale bar, 50 μm. (C) Compared the expression of HA in decidua from women with NP (n=8) or RM (n=8) by Alcian blue staining assays. Scale bar, 50 μm. (D) Levels of CD16, CD86, CD206 and CD209 in CD45 + CD14 + dMφ from women with NP (n=7) or RM (n=7) by flow cytometry assays. The data are presented as the mean ± SEM; two-tailed Student’s t -test; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Article Snippet: M2φ were treated with exogenous COL4A1 (10 ug/mL; Proteintech, Ag34402), OPN (1 ug/mL; ABclonal, RP00989), and HA (High Molecular Weight; 50μM, 100μM; R&D system, GLR002) for 48 hours.

    Techniques: Expressing, Immunohistochemistry, Staining, Flow Cytometry, Two Tailed Test